8BIO
Crystal structure of human Ephrin type-A receptor 2 (EPHA2) Kinase domain in complex with MRAL5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999990 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.840, 107.413, 40.651 |
| Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
| Resolution | 38.460 - 1.600 |
| R-factor | 0.16498 |
| Rwork | 0.163 |
| R-free | 0.19860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nka |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.321 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.430 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 227442 | 1329 |
| <I/σ(I)> | 15 | |
| Completeness [%] | 96.5 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.999 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG3350, 10% ethylene glycol, 0.2M potassium thiocyanate |
