8BGG

Cryo-EM structure of SARS-CoV-2 spike (Omicron BA.1 variant) in complex with nanobody W25 (map 5, focus refinement on RBD, W25 and adjacent NTD)

EM specimen

Vitrification	Yes
Instrument	LEICA EM GP

EM Data Collection

Microscope	JEOL CRYO ARM 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	6.04
Number of Particle	2458527
Software	RELION,cryoSPARC,PHENIX,SerialEM,CTFFIND
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION