Journal: To Be Published Title: Cryo-EM structure of SARS-CoV-2 spike (Omicron BA.1 variant) in complex with nanobody W25 (map 5, focus refinement on RBD, W25 and adjacent NTD) Authors: Schwefel D
Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 9125 / Average exposure time: 3.3 sec. / Average electron dose: 40.0 e/Å2
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Image processing
Particle selection
Number selected: 2458527
Initial angle assignment
Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.1)
Final 3D classification
Number classes: 3 / Software - Name: cryoSPARC (ver. 3.3.1)
Final angle assignment
Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.1)
Final reconstruction
Number classes used: 1 / Resolution.type: BY AUTHOR / Resolution: 6.04 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.3.1) / Number images used: 32512
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Atomic model buiding 1
Refinement
Space: REAL / Protocol: OTHER
Output model
PDB-8bgg: Cryo-EM structure of SARS-CoV-2 spike (Omicron BA.1 variant) in complex with nanobody W25 (map 5, focus refinement on RBD, W25 and adjacent NTD)
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