8BG2
Crystal structure of the SARS-CoV-2 S RBD in complex with pT1580 scFV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.046, 85.900, 88.198 |
Unit cell angles | 90.00, 117.04, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.760 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.095 | 1.210 |
Rmeas | 0.112 | 1.424 |
Rpim | 0.059 | 0.742 |
Number of reflections | 234717 | 5914 |
<I/σ(I)> | 9.19 | 1.15 |
Completeness [%] | 94.1 | 88.15 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.997 | 0.444 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | pT1580-RBD at 9,8 mg/ml using 1.4 M Na-Tartrate, 100 mM Tris pH 8.5 as reservoir |