8BFI

human CNOT1-CNOT10-CNOT11 module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	83.760, 110.640, 128.580
Unit cell angles	90.00, 103.39, 90.00

Refinement procedure

Resolution	45.770 - 3.000
R-factor	0.2631
Rwork	0.262
R-free	0.28270
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.627
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.770	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.321
Number of reflections	89839	4548
<I/σ(I)>	22.31
Completeness [%]	100.0
Redundancy	68.3
CC(1/2)	0.999	0.435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	5% (w/v) PEG 3500, 2% (v/v) isopropanol, 0.1 M MES pH 5.5