8BFB
Sarkosyl-extracted AppNL-G-F Abeta42 fibril structure (Methoxy-X04-labelled mice)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | TFS Selectris |
Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 3.2 |
Number of Particle | 136214 |
Software | crYOLO,RELION,PHENIX,EPU,CTFFIND,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |