8BEC
Crystal structure of the SARS-CoV-2 S RBD in complex with pT1375 scFV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.446, 91.559, 156.704 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.780 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.079 | 1.532 |
Rmeas | 0.082 | 1.594 |
Rpim | 0.022 | 0.435 |
Number of reflections | 138573 | 13750 |
<I/σ(I)> | 17.68 | 1.28 |
Completeness [%] | 100.0 | 99.98 |
Redundancy | 13.3 | 13.1 |
CC(1/2) | 0.999 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | pT1375-RBD at 15,5 mg/ml using 1.5 M Ammonium sulfate, 15 % w/v glycerol, 100 mM Tris pH 8.5 as reservoir and micro seeds in 18% PEG 3350, 100 mM citrate pH 4, 200 mM Na3Cit stabilization solution |