8BE5
Crystal structure of SOS1-KRasG12V-Nanobody22-Nanobody75
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96858 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 124.080, 124.080, 408.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.910 - 3.130 |
| R-factor | 0.2286 |
| Rwork | 0.226 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.381 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.320 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Number of reflections | 28523 | 4364 |
| <I/σ(I)> | 13.65 | |
| Completeness [%] | 99.3 | |
| Redundancy | 10.8 | |
| CC(1/2) | 0.990 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG 4000, 0.1 M sodium citrate, pH 5.5 and 0.2 M sodium acetate |
