8BDF

Tubulin-taxane-2a complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.99987
Spacegroup name	P 21 21 21
Unit cell lengths	104.840, 157.890, 179.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.284 - 1.950
R-factor	0.1904
Rwork	0.189
R-free	0.21570
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.284	49.284	2.000
High resolution limit [Å]	1.950	8.720	1.950
Rmerge	0.133	0.027	5.166
Rmeas	0.136	0.028	5.261
Total number of observations	5894682
Number of reflections	215803	2621	15836
<I/σ(I)>	20.05	111.21	0.73
Completeness [%]	100.0	98.7	100
Redundancy	27.315	25.325	27.761
CC(1/2)	1.000	1.000	0.314

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	2-4% PEG 4K, 2-10% glycerol, 30 mM MgCl2, 524 30 mM CaCl2, 0.1 M MES/Imidazole