8BCG

Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 86

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	99.917, 118.915, 188.069
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.060 - 2.390
R-factor	0.2153
Rwork	0.214
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s8q
RMSD bond length	0.007
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.390	2.390
Rmeas	0.215
Number of reflections	89144	14027
<I/σ(I)>	8.68	0.79
Completeness [%]	99.7	98.4
Redundancy	6.7	6.9
CC(1/2)	0.996	0.285

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	291.15	0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350