8BCF

Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 78

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	99.549, 118.698, 186.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.250 - 2.420
R-factor	0.2144
Rwork	0.213
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s8q
RMSD bond length	0.005
RMSD bond angle	0.752
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.420	2.420
Rmeas	0.231
Number of reflections	85165	13518
<I/σ(I)>	9.14	0.83
Completeness [%]	99.9	99.4
Redundancy	10.5	10.5
CC(1/2)	0.997	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350