8BCC

Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	100.425, 119.408, 188.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.610 - 2.350
R-factor	0.2109
Rwork	0.210
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s8q
RMSD bond length	0.006
RMSD bond angle	0.801
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.350	2.350
Rmeas	0.203
Number of reflections	94679	14864
<I/σ(I)>	8.55	0.95
Completeness [%]	99.6
Redundancy	6.7
CC(1/2)	0.995	0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350