8BBY

VarB H/L (SLPL/SLPH) complex from C. difficile SlpA (R20291 strain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.708459
Spacegroup name	C 1 2 1
Unit cell lengths	108.926, 131.291, 138.269
Unit cell angles	90.00, 108.78, 90.00

Refinement procedure

Resolution	36.230 - 2.900
R-factor	0.2459
Rwork	0.244
R-free	0.28690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7acz
Data reduction software	DIALS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.230	3.000
High resolution limit [Å]	2.900	2.900
Rpim	0.086	0.404
Number of reflections	40129	3584
<I/σ(I)>	8	2.19
Completeness [%]	97.8	86.49
Redundancy	2
CC(1/2)	0.978	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.03 M Magnesium chloride hexahydrate; 0.03 M Calcium chloride dihydrate, 0.12 M Ethyleneglycol, 0.05 M Tris (base); 0.05 M BICINE pH 8.5, 20% v/v Glycerol; 10% w/v PEG 4,000