8BBQ

Determination of the structure of active tyrosinase from bacterium Verrucomicrobium spinosum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-26
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97626
Spacegroup name	C 1 2 1
Unit cell lengths	84.813, 63.422, 116.020
Unit cell angles	90.00, 96.83, 90.00

Refinement procedure

Resolution	115.200 - 1.430
R-factor	0.1709
Rwork	0.169
R-free	0.20040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z11 4z12 5ce9
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.200	1.560
High resolution limit [Å]	1.430	1.430
Number of reflections	62664	3134
<I/σ(I)>	10.5
Completeness [%]	93.4
Redundancy	6.8
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M ammonium sulfate, 0.1 M MES pH 6.5, 30% w/v PEG5000MME