8B9Y
Cysteine Synthase from Trypanosoma cruzi with PLP and OAS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-11 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.006, 66.667, 167.518 |
| Unit cell angles | 90.00, 90.47, 90.00 |
Refinement procedure
| Resolution | 54.940 - 1.800 |
| Rwork | 0.191 |
| R-free | 0.21730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4air |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.362 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.940 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 110107 | 5376 |
| <I/σ(I)> | 12.93 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.37 | |
| CC(1/2) | 0.996 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium Sulfate, Bis-Tis |
