8B9U
Structure of ClpC1 NTD from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-03-21 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54789 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 57.440, 57.440, 174.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.840 - 2.250 |
R-factor | 0.2141 |
Rwork | 0.212 |
R-free | 0.24940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wdb |
RMSD bond length | 0.002 |
RMSD bond angle | 0.438 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmeas | 0.089 | 0.653 |
Number of reflections | 16532 | 1084 |
<I/σ(I)> | 19.7 | |
Completeness [%] | 98.9 | 88.2 |
Redundancy | 11.6 | 10.5 |
CC(1/2) | 0.999 | 0.933 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10 mM Tris-HCl pH 7.5 and 3.1 M sodium formate |