8B7D

Luminal domain of TMEM106B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-07
Detector	DECTRIS EIGER2 X CdTe 9M
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	52.408, 52.408, 132.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 2.590
R-factor	0.2349
Rwork	0.234
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	alpha fold model Q8N353
RMSD bond length	0.004
RMSD bond angle	0.606
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (dev_4682)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	2.640
High resolution limit [Å]	2.590	2.590
Rmerge	0.187	3.804
Rpim	0.038	0.756
Number of reflections	6291	301
<I/σ(I)>	13.5
Completeness [%]	100.0	100
Redundancy	25	25.8
CC(1/2)	0.371	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.0 M NaCl, 0.1 M BisTris-HCl, pH 5.5