8B7B

Tubulin - maytansinoid - 6 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999995
Spacegroup name	P 21 21 21
Unit cell lengths	104.085, 155.637, 181.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.830 - 2.250
R-factor	0.1858
Rwork	0.184
R-free	0.22430
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
RMSD bond length	0.002
RMSD bond angle	0.489
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.830	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.149	2.913
Rmeas	0.155	3.029
Rpim	0.041	0.824
Number of reflections	139947	13689
<I/σ(I)>	16.76	0.86
Completeness [%]	99.4	98.84
Redundancy	13.7	13.3
CC(1/2)	0.999	0.357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	5% PEG 4K, 8% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine