8B7A

Tubulin - maytansinoid - 4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000029
Spacegroup name	P 21 21 21
Unit cell lengths	105.271, 158.550, 181.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.150 - 2.250
R-factor	0.1829
Rwork	0.181
R-free	0.22300
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.150	48.150	2.310
High resolution limit [Å]	2.250	10.060	2.250
Rmerge	0.185	0.022	4.308
Rmeas	0.192	0.023	4.493
Rpim	0.052
Number of reflections	144473	1774	10477
<I/σ(I)>	14.23	85.8	0.56
Completeness [%]	99.9	98.7	98.9
Redundancy	13.6	12.731	12.47
CC(1/2)	0.999	1.000	0.201

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	5% PEG 4K, 8% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine