8B79

The crystal structure of M644G variant of DNA Pol Epsilon containing UTP in the polymerase active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97964
Spacegroup name	P 1 2 1
Unit cell lengths	154.400, 70.700, 159.400
Unit cell angles	90.00, 112.80, 90.00

Refinement procedure

Resolution	43.540 - 2.650
R-factor	0.2169
Rwork	0.215
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m8o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.540	3.600	2.800
High resolution limit [Å]	2.650	2.800	2.650
Rmerge	0.127	0.410	1.720
Rmeas	0.137	0.440	1.860
Number of reflections	92708	41372	14050
<I/σ(I)>	9.89	4.2	0.99
Completeness [%]	99.8	99.9	99.9
Redundancy	6.87	7	7.06
CC(1/2)	0.998	0.970	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	50mM MES, PEG20K,2.5% glycerol, 150mM Sodium Actetate