8B69

Heterotetramer of K-Ras4B(G12V) and Rgl2(RBD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	77.843, 77.843, 163.728
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.450 - 3.070
R-factor	0.2401
Rwork	0.237
R-free	0.31700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lfd
RMSD bond length	0.007
RMSD bond angle	1.402
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.472	3.180
High resolution limit [Å]	3.070	3.071
Rmerge	0.027	0.177
Rmeas	0.038	0.251
Rpim	0.027
Number of reflections	11107	949
<I/σ(I)>	10.15	2.11
Completeness [%]	98.2	86.51
Redundancy	2	1.9
CC(1/2)	0.999	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.2M Sodium/potassium phosphate pH 7.5, 0.1M HEPES 7.5, 22.5% v/v PEG (16.67%w/v PEG 8000, 16.67%w/v PEG 10000, 16.67% w/v PEG 6000), 10% v/v Glycerol