8B5W
Crystal structure of the E3 module from UBR4
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.2820 or 1.2831 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 67.131, 84.644, 148.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.070 - 1.800 |
R-factor | 0.1993 |
Rwork | 0.198 |
R-free | 0.22160 |
Structure solution method | MAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.543 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | CRANK2 (2.01) |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.070 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.024 | 0.291 |
Rmeas | 0.034 | 0.411 |
Rpim | 0.024 | 0.291 |
Number of reflections | 36081 | 3577 |
<I/σ(I)> | 23.84 | 2.56 |
Completeness [%] | 91.3 | 91.67 |
Redundancy | 2 | 2 |
CC(1/2) | 0.999 | 0.872 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10 mM Na2HPO4 13% PEG20000 |