8B1N
Crystal structure of TrmD-Tm1570 from Calditerrivibrio nitroreducens in complex with S-adenosyl-L-methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-30 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.780, 199.560, 56.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.300 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.138 | 1.523 |
Number of reflections | 75191 | 11921 |
<I/σ(I)> | 13.68 | 1.43 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 8.81 | 8.69 |
CC(1/2) | 0.999 | 0.616 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | Protein sample: 5.7 mg/mL TrmD-Tm1570 in 50 mM HEPES buffer pH 7.4, 300 mM NaCl, 5% glycerol, with addition of 3 mM SAM, 0.1% DDM and 3000x diluted seeds stock. Reservoir solution: 0.2 M MgCl2, 0.1 M Tris pH 7.0, 10% PEG 8000. |