8AXS
Sialidases and Fucosidases of Akkermansia muciniphila are key for rapid growth on colonic mucin and nutrient sharing amongst mucin-associated human gut microbiota
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-05 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.976254 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.070, 51.780, 118.440 |
| Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
| Resolution | 50.250 - 1.300 |
| R-factor | 0.1672 |
| Rwork | 0.166 |
| R-free | 0.18760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | alphafold model |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.224 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.135 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmeas | 0.069 | 1.000 |
| Number of reflections | 308465 | 22149 |
| <I/σ(I)> | 12.14 | 0.8 |
| Completeness [%] | 99.7 | 97.1 |
| Redundancy | 5.5 | 4 |
| CC(1/2) | 0.999 | 0.440 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 18% PEG 3350,0.1 M BIS TRIS, 0.2 M MgCl2 |
