8AXI

Sialidases and Fucosidases of Akkermansia muciniphila are key for rapid growth on colonic mucin and nutrient sharing amongst mucin-associated human gut microbiota

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976254
Spacegroup name	P 1 21 1
Unit cell lengths	103.920, 51.510, 118.470
Unit cell angles	90.00, 93.15, 90.00

Refinement procedure

Resolution	50.210 - 1.250
R-factor	0.1616
Rwork	0.161
R-free	0.17600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	alphafold model
RMSD bond length	0.007
RMSD bond angle	1.050
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.510	1.280
High resolution limit [Å]	1.250	1.250
Rmerge	0.050	0.647
Rmeas	0.054	0.713
Number of reflections	323513	17780
<I/σ(I)>	16.27	2.51
Completeness [%]	93.6	69.8
Redundancy	6.2
CC(1/2)	0.990	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	MgCl2, BIS-TRIS pH 5.5, Polyethylene glycol 3350