8AWL

Fab RVFV-268

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	54.382, 70.388, 129.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.740 - 1.620
R-factor	0.203
Rwork	0.202
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zmj
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.740	1.650
High resolution limit [Å]	1.620	1.620
Number of reflections	64027	3003
<I/σ(I)>	11.4
Completeness [%]	99.5
Redundancy	13.3
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M citric acid pH 3.5, 20% w/v polyethylene glycol (PEG) 1,500 and 4% v/v (+/-)-2-methyl-2,4-pentanediol