8AV1
Crystal structure of GSK3 beta (GSK3b) in complex with CD7.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.196, 112.106, 67.320 |
Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
Resolution | 57.280 - 2.150 |
R-factor | 0.1801 |
Rwork | 0.178 |
R-free | 0.20970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q3d |
RMSD bond length | 0.008 |
RMSD bond angle | 1.149 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 57.280 | 57.280 | 2.230 |
High resolution limit [Å] | 2.150 | 8.330 | 2.150 |
Rmerge | 0.093 | 0.055 | 0.866 |
Rmeas | 0.109 | 0.060 | 1.027 |
Rpim | 0.042 | 0.022 | 0.395 |
Number of reflections | 53104 | 948 | 5161 |
<I/σ(I)> | 11.8 | 2.1 | |
Completeness [%] | 99.0 | 99.3 | 98.7 |
Redundancy | 6.5 | 7 | 6.6 |
CC(1/2) | 0.998 | 0.997 | 0.582 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277.15 | 14% PEG 3350, 0.1 M ammonium sulfate and 0.1 M bis-tris pH 6.0 |