8ARP
Crystal structure of DEAD-box protein Dbp2 in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979300 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 145.939, 168.843, 168.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 119.400 - 3.050 |
R-factor | 0.6542 |
Rwork | 0.223 |
R-free | 0.27040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8ark |
RMSD bond length | 0.005 |
RMSD bond angle | 0.998 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 119.400 | 3.099 |
High resolution limit [Å] | 3.046 | 3.046 |
Rmerge | 0.153 | 1.284 |
Rmeas | 0.159 | 1.355 |
Rpim | 0.044 | 0.362 |
Number of reflections | 1019758 | 3975 |
<I/σ(I)> | 11.2 | 2.1 |
Completeness [%] | 96.2 | 100 |
Redundancy | 13.2 | 13.4 |
CC(1/2) | 0.998 | 0.688 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.02 M (NH4)2SO4 100 mM Tris pH 7.0 150 mM Li2SO4 |