8AQ3
In surfo structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli in complex with PE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 160.994, 160.994, 91.282 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.376 - 2.395 |
| R-factor | 0.2255 |
| Rwork | 0.225 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n6l |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3494) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.480 | 2.540 |
| High resolution limit [Å] | 2.395 | 2.400 |
| Rmeas | 0.140 | 2.920 |
| Number of reflections | 53802 | 8590 |
| <I/σ(I)> | 12.46 | 0.88 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 11.1 | 11.22 |
| CC(1/2) | 0.990 | 0.460 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 50 mM sodium acetate pH 5.0, 50 mM magnesium acetate and 28-36 %(v/v) PEG200 |
