8AOP

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 14r

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	56.790, 59.194, 87.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.700 - 1.944
Rwork	0.197
R-free	0.22720
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4v2y
RMSD bond length	0.009
RMSD bond angle	1.496
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.060
High resolution limit [Å]	1.940	1.940
Rmerge	0.048	0.979
Number of reflections	22417	6778
<I/σ(I)>	19.51	1.7
Completeness [%]	100.0	99.9
Redundancy	6.93	6.99
CC(1/2)	1.000	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.4M Ammonium Phosphate