8ANS
Crystal structure of D1228V c-MET bound by compound 1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.890, 52.830, 96.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.560 - 2.010 |
| R-factor | 0.2318 |
| Rwork | 0.229 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sdc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.060 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (24-FEB-2021)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.560 | 35.570 | 2.040 |
| High resolution limit [Å] | 2.010 | 5.450 | 2.010 |
| Rmeas | 0.169 | 0.076 | 1.456 |
| Rpim | 0.067 | 0.032 | 0.567 |
| Total number of observations | 113620 | 5669 | 5830 |
| Number of reflections | 17868 | 1006 | 891 |
| <I/σ(I)> | 6.9 | 18.1 | 1.4 |
| Completeness [%] | 99.9 | 100 | 99.9 |
| Redundancy | 6.4 | 5.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15 % PEG10K, 100 mM PCPT pH 7.5 |
