8ALX
Structure of human PD-L1 in complex with inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-18 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 56.345, 65.473, 78.591 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.710 - 1.100 |
R-factor | 0.118 |
Rwork | 0.117 |
R-free | 0.13600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5c3t |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.070 | 1.160 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.047 | 0.299 |
Number of reflections | 58363 | 7707 |
<I/σ(I)> | 16.3 | |
Completeness [%] | 98.6 | |
Redundancy | 5.7 | |
CC(1/2) | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.4 M sodium acetate trihydrate; 0.1 M sodium cacodylate pH 6.5 |