8AHY

X-ray structure of human NCS-1 bound to Ric-8A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979263
Spacegroup name	P 41 21 2
Unit cell lengths	56.635, 56.635, 135.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.240 - 1.700
R-factor	0.1874
Rwork	0.186
R-free	0.20760
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	0.774
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.240	1.784
High resolution limit [Å]	1.700	1.704
Number of reflections	44184	1101
<I/σ(I)>	13.6
Completeness [%]	96.0	66.4
Redundancy	25.5
CC(1/2)	0.997	0.538

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	30 % PEG 4000, 100 mM sodium acetate pH 4.6, 100 mM magnesium chloride