8AHT
Crystal structure of Plasmodium falciparum Ca2+/Calmodulin in complex with melittin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-22 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 37.900, 60.680, 68.450 |
Unit cell angles | 95.77, 89.99, 97.05 |
Refinement procedure
Resolution | 47.430 - 2.200 |
R-factor | 0.2356 |
Rwork | 0.234 |
R-free | 0.26610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cdl |
RMSD bond length | 0.003 |
RMSD bond angle | 0.543 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.430 | 2.400 | 2.300 |
High resolution limit [Å] | 2.200 | 2.300 | 2.200 |
Rmeas | 0.090 | 0.624 | 1.017 |
Number of reflections | 28647 | 3089 | 3250 |
<I/σ(I)> | 8.78 | 1.8 | 1.25 |
Completeness [%] | 93.5 | ||
Redundancy | 1.796 | ||
CC(1/2) | 0.998 | 0.768 | 0.832 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.25M NaCl, 30w/v% PEG3350, 0.1M Bis-Tris buffer, pH 6.0 |