8AF0

Crystal structure of human angiogenin and RNA duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	50.380, 66.660, 101.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.120 - 2.430
R-factor	0.2083
Rwork	0.205
R-free	0.26810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eop
RMSD bond length	0.010
RMSD bond angle	1.442
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.120	2.530
High resolution limit [Å]	2.430	2.430
Number of reflections	24805	2830
<I/σ(I)>	13.03	1.49
Completeness [%]	99.8	99.8
Redundancy	7.088
CC(1/2)	0.999	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	200 mM NaCl, 20 % (w/v) PEG6000, 100 mM Hepes pH 7.0