8AEZ
X-ray structure of the deglycosylated receptor binding domain of Env glycoprotein of Simian Foamy virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 99.451, 99.451, 120.609 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.730 - 2.574 |
R-factor | 0.2153 |
Rwork | 0.213 |
R-free | 0.25300 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.980 |
Data reduction software | XDS (`) |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.730 | 2.666 |
High resolution limit [Å] | 2.574 | 2.574 |
Rmerge | 0.205 | 0.936 |
Rpim | 0.047 | 0.212 |
Number of reflections | 22363 | 2101 |
<I/σ(I)> | 10.1 | 1.85 |
Completeness [%] | 99.9 | |
Redundancy | 20.3 | |
CC(1/2) | 0.986 | 0.846 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.1M Tris pH 8.5, 3.5M sodium formate |