8ACI
Structure of ARG-117 Fab in complex with a fragment of complement C2, neutral pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.590, 88.760, 144.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.160 - 1.850 |
| R-factor | 0.1877 |
| Rwork | 0.187 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ani |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.975 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.160 | 1.916 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.139 | 4.450 |
| Number of reflections | 79409 | 7809 |
| <I/σ(I)> | 19.68 | |
| Completeness [%] | 100.0 | 99.99 |
| Redundancy | 52.1 | 44.1 |
| CC(1/2) | 1.000 | 0.562 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | The reservoirs contained 50-70% MPD, 100 mM Hepes pH 7.0-7.4 |
