8A9R

Tetragonal Hen Egg-White (HEW) Lysozyme soaked in reduced resazurin in a glovebox and flash-cooled using a miniature-airlock

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 43 21 2
Unit cell lengths	77.229, 77.229, 36.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.000 - 1.600
Rwork	0.168
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xbr
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.700
High resolution limit [Å]	1.590	1.590
Rmeas	0.093	1.270
Number of reflections	15200	2299
<I/σ(I)>	10.4	1.14
Completeness [%]	98.8
Redundancy	6.5
CC(1/2)	0.990	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM sodium acetate pH 4.6, 1M NaCl