8A6E
100 picosecond light activated crystal structure of bovine rhodopsin in Lipidic Cubic Phase (SACLA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 294 |
Detector technology | CCD |
Collection date | 2018-11-01 |
Detector | MPCCD |
Wavelength(s) | 1.24 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 61.290, 90.810, 150.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.470 - 1.800 |
R-factor | 0.332 |
Rwork | 0.332 |
R-free | 0.36920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u19 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.623 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.470 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 78209 | 7715 |
<I/σ(I)> | 6.2 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 679.1 | 487.5 |
CC(1/2) | 0.995 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 9 | 294 | 36 % PEG 600, 100 mM Bicine pH 9.0 |