8A5N
X-ray structure of human H-chain ferritin treated with SDS
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.920, 183.920, 183.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 106.180 - 1.520 |
| Rwork | 0.151 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n27 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.815 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.180 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.185 | |
| Rmeas | 0.185 | |
| Rpim | 0.016 | |
| Number of reflections | 41266 | 2030 |
| <I/σ(I)> | 41.2 | 2.4 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 20 | 15 |
| CC(1/2) | 1.000 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 2.0 M magnesium chloride 0.1 M bicine buffer pH 9.0 |
