8A4K

Human GDAP1, R282H mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-27
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.03319
Spacegroup name	P 21 21 21
Unit cell lengths	73.279, 113.370, 115.194
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.840 - 1.950
R-factor	0.2254
Rwork	0.225
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7alm
RMSD bond length	0.014
RMSD bond angle	1.371
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	1.950	1.950
Rmerge	0.049	2.738
Rmeas	0.053	2.969
Number of reflections	70450	11256
<I/σ(I)>	15.4	0.7
Completeness [%]	99.8	99.9
Redundancy	6.7
CC(1/2)	0.999	0.416

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M succinic acid; 20% (w/v) PEG 3350