8A4C

Structure of human Rep15:Rab3B complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999961
Spacegroup name	C 1 2 1
Unit cell lengths	188.810, 59.220, 109.020
Unit cell angles	90.00, 111.90, 90.00

Refinement procedure

Resolution	47.100 - 2.750
R-factor	0.22
Rwork	0.218
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8a4b
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.100	2.848
High resolution limit [Å]	2.750	2.750
Rmeas	0.144
Rpim	0.055	0.673
Number of reflections	29437	2888
<I/σ(I)>	14.13	1.98
Completeness [%]	99.8
Redundancy	6.7
CC(1/2)	0.998	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Bis-Tris pH 7.5, 0.2 M NaI and 20% (w/v) PEG3350