8A48
Less crystallisable" IgG1 Fc fragment (E382S variant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-18 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 106.780, 106.780, 104.011 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.543 - 3.044 |
| Rwork | 0.197 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ave |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.797 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.543 | 3.250 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.034 | 1.117 |
| Rmeas | 0.048 | 1.580 |
| Rpim | 0.034 | 1.117 |
| Number of reflections | 13476 | 2358 |
| <I/σ(I)> | 15.2 | 0.6 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | 0.2 M ammonium sulphate, 0.1 M Tris (pH 7.5), 25 % w/v PEG 8000 |
