8A29
Apo 1-deoxy-D-xylulose 5-phosphate synthase from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-27 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 116.444, 137.631, 232.082 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.590 - 2.100 |
R-factor | 0.1733 |
Rwork | 0.171 |
R-free | 0.22390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ouv |
RMSD bond length | 0.011 |
RMSD bond angle | 1.062 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.590 | 2.279 |
High resolution limit [Å] | 2.100 | 2.200 |
Rmerge | 0.058 | 0.294 |
Number of reflections | 216635 | 18663 |
<I/σ(I)> | 11.09 | |
Completeness [%] | 99.9 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM HEPES, 12% PEG 8000, 200 mM calcium acetate |