8A1F
Human PTPRK N-terminal domains MAM-Ig-FN1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.790, 93.862, 181.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.460 - 3.000 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.27380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5y |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.735 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.050 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 30882 | 1502 |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.3 | |
| CC(1/2) | 1.000 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM HEPES, pH 7, 10% (w/v) polyethylene glycol (PEG) 6000 |
