8A17

Human PTPRM domains FN3-4, in spacegroup P3221

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-17
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9763
Spacegroup name	P 32 2 1
Unit cell lengths	87.531, 87.531, 311.235
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	75.800 - 3.090
R-factor	0.2458
Rwork	0.243
R-free	0.28970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v5y
RMSD bond length	0.012
RMSD bond angle	1.360
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.890	3.140
High resolution limit [Å]	3.080	3.080
Number of reflections	26535	1199
<I/σ(I)>	9.1
Completeness [%]	99.8
Redundancy	10
CC(1/2)	0.993	0.391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM ammonium nitrate and 10% PEG 3350