8A12

Plasmodium falciparum Myosin A full-length, post-rigor state complexed to Mg.ATP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-02-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96770
Spacegroup name	P 21 21 21
Unit cell lengths	90.125, 115.140, 170.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.560 - 2.030
R-factor	0.1882
Rwork	0.186
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ycy
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.112	2.223
High resolution limit [Å]	2.027	2.027
Rmerge	0.078	1.136
Number of reflections	87835	4371
<I/σ(I)>	13.1
Completeness [%]	94.4
Redundancy	6.9
CC(1/2)	0.999	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277.4	1,6 M Ammonium sulfate; 0,1 M HEPES pH 7,5; 4% PEG400