8Z54

Crystal structure of human SIRT5 in complex with succinylated Prx1 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	45.202, 72.612, 162.407
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.310 - 1.450
R-factor	0.2032
Rwork	0.202
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.407
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.370	1.500
High resolution limit [Å]	1.450	1.450
Rmeas	0.039	0.461
Number of reflections	47857	4633
<I/σ(I)>	20.1	3.1
Completeness [%]	99.5
Redundancy	4.61
CC(1/2)	0.999	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	24% PEG 3350, 0.1M Tris-HCl pH 8.5