8Z50

Crystal structure of the ASF1-H3T-H4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	96.940, 103.873, 85.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.960 - 2.800
R-factor	0.2092
Rwork	0.206
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.621
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.117	0.057	0.599
Rmeas	0.125	0.061	0.642
Rpim	0.043	0.022	0.228
Total number of observations	92323
Number of reflections	11137	620	553
<I/σ(I)>	5.6
Completeness [%]	100.0	99.5	100
Redundancy	8.3	7.5	7.6
CC(1/2)	0.999	0.996	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.0 M Lithium chloride, 0.1 M MES pH 6.0, 10% PEG 6000