8YWR
Crystal structure of the Fab fragment of the anti-IL-6 antibody 68F2 in complex with a domain-swapped IL-6 dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.971 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 46.550, 92.860, 84.630 |
| Unit cell angles | 90.00, 103.93, 90.00 |
Refinement procedure
| Resolution | 41.070 - 2.930 |
| R-factor | 0.2183 |
| Rwork | 0.214 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zs7 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.511 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.000 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Rmerge | 0.180 | 0.870 |
| Number of reflections | 14778 | 969 |
| <I/σ(I)> | 7.5 | 2.3 |
| Completeness [%] | 97.9 | 88.8 |
| Redundancy | 6.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG 4K, 0.15 M (NH4)2SO4, 0.1 M MES |
